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KMID : 1007520140230020569
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Food Science and Biotechnology
2014 Volume.23 No. 2 p.569 ~ p.575
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Preparation, Characterization, and In vitro Antitumor Activity of Folate Conjugated Chitosan Coated EGCG Nanoparticles
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Liang Jin
Cao Lin
Zhang Liang
Wan Xiao-chun
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Abstract
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Folate conjugated chitosan coated EGCG nanoparticles (FCS-EGCG-NPs) were prepared using the ionic gelation method with folic acid modified carboxymethyl chitosan (FA-CMC) and chitosan hydrochloride as carriers of catechin EGCG. Characteristics of FCS-EGCG-NPs were determined using transmission electron microscopy (TEM) and fourier transform infrared spectroscopy (FTIR). Synthesized FCS-EGCG-NPs were spherical in shape with a mean diameter of 400 nm. The maximum encapsulation rate of nanoparticles loaded with EGCG was 75%. FTIR spectra suggested formation of an amide linkage between carboxyl groups of FA-CMC and the amine groups of chitosan hydrochloride. FCS-EGCG-NPs demonstrated sustained release of EGCG in buffer solutions of different pH values. The antitumor activity of FCS-EGCG-NPs towards different cancer cells was also investigated. FCSEGCG- NPs had a greater tumor inhibition effect on cancer cells having a large expression of folic acid receptors on the surface than cancer cells with lesser expression.
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KEYWORD
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folate, chitosan, catechin, nanoparticles, antitumor
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